Product: Hy-Start Hotstart Mix (x2)
2× Hy Start Hotstart Mix is a premixed, ready to use solution containing Hotstart Taq DNA Polymerase, dNTPs, Mg 2+ and Reaction Buffer at optimal concentrations for efficient
amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetti ng steps required for PCR set up. The mix retains all features of Hotstart Taq DNA Polymerase.
Hy-Start Hotstart Taq DNA Polymerase is a hot start polymerase with chemical modification, which brings higher specificity by reducing non specific products as the enzyme activity is temperature dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 2min/kb (simple template up to 20s/kb) separately. Hotstart Taq has 5 3 polymerase activity, but no 3 5 exonuclease activity. The product of Hotstart Taq has overhanged dA at 3 end.
Hy-start Hotstart Taq DNA Polymerase has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot start polymerase. Its efficiency is higher than most chemical modified polymerases and the initial denaturation time can be reduced to 3 minutes.