Product: DQP – Double Quenched Probes
Double quenched probes (DQPs) are optimized for use in real-time quantitative PCR and are designed to efficiently reduce background noise in probes longer than 25 nts. The length of the linear hydrolysis probe used in 5′ nuclease assays is carefully designed to balance quenching efficiency and binding stability. Sometimes it is necessary to extend the probe sequence beyond the 20-30 nt window to achieve the necessary probe Tm, especially for AT-rich target sequences. However, increasing the sequence length can diminish the quenching efficiency and result in poor signal-to-noise values. To address this, an internal quencher can be introduced closer to the fluorescent 5′ moiety to reduce background fluorescence, and an additional 3′ quencher can act as a Polymerase inhibitor to prevent the probe from being extended upon hybridization. Hylabs’ range of reporter-quencher combinations offered in their portfolio are suitable for single or multiplex real-time PCR applications and can include “non-natural” nucleotides such as LNA moieties. Like their DLPs, hylabs offers their DQPs in final yields of nmoles delivered with a transparent and best price-performance scheme for their broad selection of DQPs.