Viral Detection

Specific viral detection (GMP certified)

Our method for detection of specific viral targets using Real-time PCR is based on TaqMan technology so that each virus is detected using a specific set of primers and probe which enables rapid screening and sensitive detection of low levels contaminants (<25 copies per reaction).
Panel of pathogenic viruses is presented below. Additional custom-made assays are available upon request.

List of specific viral qPCR assays

Virus Target Limit of Detection Sample Requirements
Human Immunodeficiency Virus types 1 and 2 (HIV-1, HIV-2) 10 copies Per testing of 8 DNA viral targets:
1 vial of 1ml of non-cell-based
sample or  1-1×10(7) cells per 1ml
or as pellet
Human Papillomavirus types 16 and 18 (HPV-16, HPV-18) 10 copies
Human T-Lymphotropic Virus DNA types 1 and 2 (HTLV-1, HTLV-2) 10 copies
Simian Immunodeficiency Virus (SIV) 10 copies
Mouse Minute virus (MMV) 25 copies
Bovine Polyomavirus (BPyV) 10 copies
Hepatitis B Virus (HBV) 10 copies
Parvovirus B19 (B19) 10 copies
Epstein-Barr Virus (EBV) 10 copies
Human Cytomegalovirus/Herpes Type 5 (hCMV/hHV5) 10 copies
Human Herpesvirus Type 6 (hHV6) 10 copies
Human Herpesvirus Type 7 (hHV7) 10 copies
Human Herpesvirus Type 8 (hHV8) 10 copies
Herpes Simplex Virus types 1 and 2 (HSV-1, HSV-2) 10 copies
Human/Simian Foamy Virus (HFV/SFV) 10 copies
Hepatitis C (HCV) 25 copies

Per testing of 6 RNA viral targets:
1 vial of 1ml of non-cell-based
sample or 1-1×10(7) cells per 1ml
or as pellet

Hepatitis A (HCV) 25 copies
Hepatitis E (HCV) 25 copies

Detection of retroviral activity (GMP certified)

Detection of retroviral contamination in cell-free samples is achieved by the detection of reverse transcriptase (RT) activity using PCR-Based Reverse Transcriptase assay (PBRT, also known as Amp-RT, PERT). The assay is based on RT activity-dependent conversion of target RNA into cDNA that is then detected by quantitative PCR (qPCR).

For more information: Roni Cohen: project-mbc@hylabs.co.il | 054-6565959

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