|
Molecular Genetic Assay for Combined Identification of Periodontopathogenic Bacterial Species
Periodontitis is an Infectious Disease and it is now certain that a definite group of highly pathogenic bacteria is the primary cause of progressive periodontal diseases. This leads to the need for specific diagnostics and therapies of the causative bacteria especially in cases of chronic progressive, therapy-resistant and aggressive periodontal diseases.
Purely mechanical treatment will be, by itself, unsuccessful where these tissue-invasive periodontal pathogens are present in the oral cavity, but rather antibiotic methods must be employed. Knowledge of the bacterial spectrum is therefore a prerequisite for choosing an appropriate antibacterial agent.
micro-IDent and micro-IDent plus test allows the combined molecular genetic identification of periodontopathogenic bacterial species.
|
micro-IDent assay |
micro-IDent plus assay |
|
Actinobacillus actinomycetemcomitans
Porphyromonas gingivalis
Bacteroides forsythensis
Prevotella intermedia
Treponema denticola |
Peptostreptococcus micros
Fusobacterium nucleatum
Campylobacter rectus
Eubacterium nodatum
Eikenella corrodens
Capnocytophaga sp |
The whole procedure is divided into three steps: DNA isolation from subgingival samples, a multiplex amplification with biotinylated primers and a reverse hybridization.
Instructions for Taking a Specimen
The specimen is taken in the dental practice. The following points should be observed:
1. Prior to sampling, the supra-gingival plaque should be removed with a sterile curette and the site of sampling be dried with a sterile cotton roll.
2. Principally sampling should be done prior to mechanical treatment of the pocket. Whereas contamination with blood does not affect the test result, the sampling of severely inflamed pockets which are producing pus should be avoided.
3. Using a pair of sterile forceps insert one paper point at a time into the pre-defined sites down to the base of the sulcus.
4. Leave the paper point in this position for 10 seconds.
5. For an assessing therapeutically success, a “multi-site sample” is recommended. In this case up to five paper points with swipes from single gingival pockets can be pooled in the transfer tube.
Transport of Samples
Since the assay is based on the analysis of nucleic acids no special precautions are required during transport. However, transportation taking several days (e.g. over a weekend) should be avoided, especially during summer. Store the sample in a refrigerator until rapid transport can be ensured.
|
