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Detection and Genotyping Human Papillomavirus by DNA-Based Methods
About Human papillomavirus (HPV)
wHPV is a group of viruses that includes more than 100 different types. More than 30 of these viruses are sexually transmitted and can infect the genital area of men and women.
wAbout 75% of women will become infected with one or more of the sexually transmitted HPV types at some point during adulthood. Most of them will not have any symptoms and will clear the infection on their own
Traditional Testing
wAbout a dozen HPV types (including types 16, 18, 31 and 45), called "high-risk" types, may cause abnormal Pap tests and may also lead to cancer of the cervix, vulva, vagina, anus, or penis.
wThe "low-risk" virus types (including types 6 and 11) may cause mild Pap test abnormalities or genital warts.
wInitial screening for HPV is based on the Pap testing which is a simple cytological test. However, it shows very low sensitivity and cannot predict oncogenic potential as its main limitation.
Higher sensitivity testing
wPCR-based testing has allowed the direct detection of HPV even at low-level virus copy numbers.
Hylabs offers two approaches for HPV detection and genotyping
by DNA-Based Methods:
1) Two-step analysis:
i) Nested-PCR for the detection of HPV DNA
ii) Genotyping of the HPV DNA by sequencing method
2) Single-step analysis:
i)Multiplex PCR analysis for detection and genotyping that can simultaneously screen and identify 18 different HPV types in just one tube.
*From a clinical specimen. Lab services are provided by Hylabs clinical diagnostic lab (recognized by the Israeli authority)
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