Genotyping

DNA sequence variations are used to provide insight into population dynamics, pharmacogenetics and disease gene mapping. Hy Laboratories offers a full range of genotyping services that can be scaled from regional genotyping to genome-wide screening.

Fragment Analysis & Short Tandem Repeat (STR) Analysis

Amplification reactions are performed by labeling one end of a PCR fragment with a fluorescent tag. This is achieved by using end labeled primer in the PCR reaction. As the fluorescent DNA fragment passes the point of detection on the 3100 DNA sequencer, signals are sent to a macintosh and collected by ABI’s Gene Scanner Data Collection software. In minutes the Gene Scanner Analysis software processes the gel image and interprets the data into a colored densitometric scan. Internal size standards run in each lane so that the software can automatically calculate the size of each fragment.

-     Five-dye fluorescent DNA technology enables high-throughput analysis and maintains desirable small amplicons. Possible dye: 6-FAM, VIC, NED, PET, LIZ & ROX.

Figure 1: Short Tandem Repeat Analysis of three different samples